spectramax 340. 2 M DEEMM were mixed in a microtube,. spectramax 340

 
2 M DEEMM were mixed in a microtube,spectramax 340  The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates

Features showcased by this model include temperature control, a robotics-compatible interface and Data Analysis Software. 25–0. , Sunnyvale, CA, USA) at a wavelength of 450 nm. -1 activity was determined MMP using an MMP-1 immunoassay kit (dY901; R&d Systems, Inc. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Call 1-800-745-2710. The VersaMax and SpectraMax 190 read 96-well microplates. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. 6) and 0. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. We also offer depot Repair Service from our base of operations in Southern California (San Diego). Description 1. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax 384 Plus lets you get absorbance measurements from 190 to 1000 nm quickly for samples in test tubes, cuvettes, and 96- or 384-well microplates. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). With optimized reagents and the industry leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax to perfectly match your needs. Warranty. 4. The values were corrected for nonspecific background by subtracting the OD 650. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. On-site Repair Service available nationwide for Molecular Devices Spectramax 340 Plate Reader's. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. Percent Transmission Absorbance Competitor 0. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. SPECTRAmax 340PC 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. It includes both a lamp and a new lamp holder. IMAP ® Technology from Molecular Devices enables rapid, homogeneous, and non-radioactive assay of kinases, phosphatases, and phosphodiesterases and is suited for both assay development and high-throughput screening. Endpoint, kinetic, spectrum. Molecular Devices SpectraMAX 340 Microplate Reader. Intra- and inter-assay coefficients of variation were lower than 8. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 7. The screening was initiated by adding 10 μL p-nitrophenyl phosphate (pNPP), and the catalysis of pNPP (1 mM) was continuously monitored on SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 405 nm for 20 min at 37 °C. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. The plate was covered and incubated for one hour at room temperature. Nitrocefin hydrolysis was monitored at 482 nm on a SpectraMax 340 UV-visible plate reader for high-throughput assays and an HP8453 UV-visible spectrophotometer for the low-throughput assays. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the enhanced TRF detection module for the SpectraMax iD5 Multi-Mode Microplate ReaderThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Adhesion molecule expression To determine whether baicalein could modify the oxLDL-induced adhesion molecule expression, HUVECs were grown to confluence, pretreated with baicalein for 2 hrs and stimulated with oxLDL (150 μg/ml) for. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Molecular Device M2e. Create custom fluorescence assays without having to purchase expensive filter sets. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. SpectraMAX 340 microtiter plate reader. Analytical Data Regression and Acceptance Limits The conversion of the optical density units for the test samples, VSs, and QCs to concentrations was performed using the Watson LIMS software (version. Hydrogen peroxide (H 2 O 2) was used to establish an intracellular oxidative. 1 mL of culture media with 10% FBS. 304 signal level saturation. 1x10 5 transduced, GFP + SK-NEP-1. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Microplate Readers. SpectraMax Multi-Mode Microplate Readers. Incubation with TMB substrate was done for 18 min. The SpectraMax® iD3 Multi Mode Microplate . 0 3. Operators can conveniently select up to 6 wavelengths at a time for absorbance detection in the visible wavelength range 340 nm - 850 nm. Includes: Computer Loaded with Softmax Pro 5. Molecular Device SpectraMax M3 Plate Reader - Certified with Warranty. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. Subsequently, the oxidative burst was initiated by the addition of PMA (final concentration 100 nM) and the cells incubated for 120 min at 37°C. reader (Molecular Devi ces). StakMax Microplate Handling Stacker. IC 50 values were determined by mathematical curve-fitting (SoftMaxPro Molecular Devices) and were defined as the drug concentration leading to 50% inhibition of net cell mass. SpectraMax Injector Cartridge with SmartInject™ Technology. Statistical analysis. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 314 low lamp level warning. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Wells were washed three times. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. Key benefits • User-upgradeable application modules including cellular imaging SpectraTest ABS1 Absorbance Validation Plate. Fully tested with Warranty. The intensity of red color formed in the assay was measured at a wavelength of 490 nm with an ELISA reader (SpectraMax 340, Molecular Device Co. 00. • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. The mixture was centrifuged for 10 min at 10,000 rpm, and BSA concentration in the supernatant was determined using bicinchoninic acid (BCA) assay. Figure 1. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. With a wide range of on-board features and capabilities, thermoax is ideally suited for use in modern laboratories involved in many applications. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance platereader (Molecular Devices, Union City, CA, United States). The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Microplate Readers. Briefly, cells were seeded into a. All assays were performed in triplicate, and the absorbance at 405 nm was measured using a SpectraMax 340 multiwell plate reader (Molecular Devices). When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices VersaMax Absorbance Microplate Reader, 340-850 nm, Molecular Devices (CAT#: STEM-SPO-0005-YJL) Labsystems Fluoroskan II Fluorescent Microplate Reader, 320 – 700 nm. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. The inhibition of ALDH activity by compounds and the IC50 curves were determined by measuring the formation of NAD(P)H spectrophotometrically at 340 nm (molar extinction coefficient of 6200 M −1 cm −1) on a Beckman DU-640 as well as a Spectramax 340 PC spectrophotometer (GMI, Ramsey, NJ, USA) using a purified. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. #ABS PLUS) Methods. Molecular Devices SpectraMax Gemini XS Microplate Reader, 250-850nm, Molecular Devices, for detection of samples in microplates. SpectraMax Microplate Readers. SpectraMax® M2/M2e user guide a multi-detection microplate reader with two-mode cuvette port SpectraMax M2e user guide cover 1 1 4/21/06 9:53:28 AM. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. Glutathione reductase (GR) activity was measured, according to Smith et al. control. S. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). The optical density (OD) at the wavelength of 450 nm was measured with a spectrometer (SpectraMax 340, Sunnyvale, CA, USA). ScanLater Western Blot Detection Cartridge. 1% PEG 8000 at 37°C. Molecular Devices Spectramax 340PC instructions. ” SpectraMax® Quant™ dsDNA Assay Kits The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. SpectraMax Multi-Mode Microplate Readers. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. Oxidative stress induction by H 2 O. 00 USD. The centrifuged solution was transferred to a different microtitre plate and the optical density (OD) of the change in color was measured using a plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. This reader only allows high performance HTRF measurement when assays are run in. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. Summary of Contents for Molecular Devices SPECTRAmax 340PC. Plates were washed with 1× KP solution and blocked with. Acceptor filter: 665/10 nm. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode Opening the Drawer Manually on page The SpectraMax ABS can accommodate standard 96-well plates. Customer Transformation Hub. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. TheSpectraMax®Plus384addstheabilitytoreadcuvettes. The supernatants were mixed with an LDH assay reagent, which was purchased from Takara Bio Inc. , Fremont, CA, USA). Nitric Oxide Assay. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. The OD for each serum is adjusted by subtracting either the OD for spontaneous mediator release or antigen-specific mediator release. 5, 2 mM pNPP, and recombinant enzymes, PTP1B activities were continuously monitored on a SpectraMax 340 Microplate Reader (SpectraMax, Silicon Valley, CA, USA) at 405 nm for 2 min at 30°C. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). One plate carrier adapter is provided with the instrument. Absorbance at 490 nm was determined using a SPECTRAmax 340 (Molecular Devices Corporation). 190–850 nm <± 1. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Microplate Readers. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. Values were determined according to a standard curve (log/log curve-fit). (1993). After the addition of sPLA2 (20 μ g), the reaction mixture was incubated for 40 min at 37°C, and the absorbance was read at 10 min intervals. All absorbance measurements were taken on a SpectraMax 340 PC reader equipped with SoFTMax Pro software. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). SpectraMax ® Plus 384 optics. To correct for inter. well, and strip-well microplates. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. The VersaMax and SpectraMax 190 read 96-well microplates. This was followed by an additional incubation at 37 °C for 30–60 min. chen@case. The absorbance was measured at 405 nm on a SpectraMax 340 (Molecular Devices). All assays were done in triplicate with readings at 60-s intervals using a SpectraMax 340 multiwell plate reader (Molecular Devices, San Jose, CA, United States). Our absorbance plate readers feature our PathCheck Sensor technology and. 4. Acceptor: 665nm. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. 04. using SpectraMax i3 (Molecular devices, San Jose, USA). Emission. Statistical analysis The significant differences between the different strains in the adherence assays were analyzed using the Student’s t -test. SpectraMax 340 Absorbance Microplate Reader, Molecular Devices (CAT#: STEM-SPO-0021-ZJF) Labsystems Fluoroskan II Fluorescent Microplate Reader, Excitation Wavelength Range 320 – 700 nm, Labsystems (CAT#: STEM-SPO-0006-LGZ) BioTek MQX200 Microplate Reader Spectrophotometer, 24VDC, BioTek Instruments, Inc (CAT#: STEM. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. CSF-1, IL-34 and calprotectin levels were analysed by ELISA according to instructions provided by the manufacturer (R&D Systems, Minneapolis, Minnesota, USA). , San Jose, CA) using Softmax Pro 7 software. Donor filter: 620 nm. Polystyrene plates (384-well) were precoated overnight at 4°C with 2 μg/ml of murine, human, or chimeric RANKL. Softmax PRO v5. , Sunnyvale, Calif. These lab workhorses are used in drug discovery, research, bioassay validation, and. After adding the stop solution, the absorbance at 450 nm with a wavelength correction at 540 nm was measured with a SpectraMax 340 plate reader (Molecular Devices, San Jose, CA, USA). One plate carrier adapter is provided with the instrument. The absorbances were being measured at 515 nm. SpectraMAX 340 plate reader (Molecular Devices). Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. SoftMax Pro. Confocal laser scanning microscopy (CLSM) Biofilms were formed on glass-bottomed Petri dishes (CELLview™; Greiner Bio-One GmbH, Frickenhausen, Germany) in the presence or absence (control biofilms) of the BIC 50 of antifungals as described above. 00. 0. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Briefly, cells were sequentially treated with terminal deoxynucleotidyl. the SpectraMax iD3 is a fantastic plate reader for a. Top and bottom reads are available for fluorescence, time-resolved fluorescence and luminescence detection. Briefly, ear or dorsal skin was dissected and fixed with 10% phosphate buffered formalin for 24–72 h. In vivo anti-malarial assay in mice. 2 Chemical Reagents 1. Percent viability was calculated by comparison with the control group. Plasma glucose levels were measured using FreeStyle Lite (Abbott Diabetes Care Ltd. No power cord or accessories included. After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. , Sunnyvale CA). HTRF-compatible SpectraMax Multi-Mode Readers. The loading equaled the mass of BSA per mass of particles. Studies of the regulation of AoSMC migration by Angpt2 were preformed using a wound healing assay. ELISAs were read with a Spectramax 340 from Molecular Devices (Sunnyvale, CA, USA). Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. Awareness Technology Inc. The reaction mixture was incubated for 5 min. NHDFs were seeded in microtiter plates (96 wells) at a density of 1x105 cells per well. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. Molecular Devices Spectramax 340 Plate Reader Repair. Awareness Technology, Inc. LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). . Optical density was measured using a SpectraMAX 340 (Molecular Devices, Sunnyvale, CA). ATI Note: #1: The N-34-MLA is a replacement assembly which replaces an OEM lamp. Sandwich ELISAs were used to measure insulin (DY8056, R&D Systems, Minneapolis, MN, USA). Emission. NO release assayThe SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. SPECTRAmax™ 340 (Molecular Device, CA, USA) at 37 ° C for 30 min. The SPECTRAmax 340PC can measure. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. Water – distilled water or equivalent. The reduction of HED in the presence of NADPH and GR was recorded at 340 nm. 2. The control panel consists of a 2-x-20-character LCD and eleven pressure-sensitive membrane keys that can be used to control some functions of the instrument. IMAP assays are based on binding of phosphate to immobilized metal coordination complexes on nanoparticles. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 30. The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). The microtiter plate was then incubated in the dark for 2 h at 37°C. edu Contact Phone: 216-368-8962. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 304 signal level saturation. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time-resolved fluorescence (TRF), FRET, and AlphaScreen. This is a used Molecular Devices SpectraMax 340PC Microplate Spectrophotometer. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. 3T3 cells were cultured in DMEM supplemented with 10% FCS and 1% antibiotics (10,000 units/ml penicillin and 10,000 μg/ml streptomycin in 0. 6. Immunofluorescence and Aggregation Analysis. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. density was measured at 570 nm (SpectraMax 340, Molecular De-vices, Sunnyvale, CA). Urinary proteins were measured using a Micro BCA assay kit following the manufacturer’s instructions (Thermo Fisher Scientific, Rockford, IL, USA). Quick View. Tunable for filterless flexibility. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. The SpectraMax GEMINI EM cannot be operated without the computer and SoftMax Pro software. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The LDH level in the lavage fluid was calculated based on the standard curve, nor-malized with protein concentration and expressed as ng/mg protein. 0 2. The first time you replace an OEM lamp you must use the N-34-MLA. A 0. Although it has been proven to be effective in various neurologic disorders, the mechanism of the treatment of traumatic brain injury (TBI) is not fully understood. Molecular Devices SPECTRAmax. Streamline GLP/GMP compliance. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Perkin Elmer Wallac Victor2 1420 Multilabel Counter $1,500. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. TDP-43 stress granular cells were treated with 70% ice-cold. Stat Fax 4700 Microstrip Reader. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. , Sunnyvale, CA). 2. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). Features showcased by this model include. SpectraMax Multi-Mode Microplate Readers. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. The values were compared against a standard curve that was generated using known concentrations of IL6 to calculate concentration in the. Cell viability was determined using the blue formazan assay in which colorless 3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) is metabolized to a blue product by mitochondrial dehydrogenases. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). MOLECULAR DEVICE M5. Filipin StainingSpectraMax® 340PC384 microplate reader provides everything needed to measure absorbance in the visible range, including temperature control, a robotics-compatible interface and SoftMax® Pro data analysis software. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The SPECTRAmax 340PC can measure. Total Cell Count in BALF Cells collected from the lavage fluid were suspended in 1 mL of F-12 media supplemented with 10% FBS. Western blot. Assay setup for a 384-well low-volume plate. Other technologies such as imaging. Data System. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. The optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. Donor filter: 616/10 nm. Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. Protein electrophoresis system (BioRad, Hercules, CA) and Odyssey Infrared Imaging System (Li-Cor Biosciences, Lincoln, NE) were used in western blot. 5 mL of 1 M acetate buffer (pH 4. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. , Sunnyvale, CA, USA) at 595 nm. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Call 1-800-745-2710. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. 5. VersaMax reads 96-well and strip-well plates. SpectraMax Multi-Mode Microplate Readers. Data were expressed as the mean percent viable cell vs. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. The release of p-nitrophenol ( 400) 17000 M-1 cm-1) was monitored at 25 °C for the enzymatic hydrolysis of com-pounds 1-8. The plate was read on a SpectraMax 340 PC plate reader (Molecular Devices, Sunnyvale, CA) at 450 nm, with reference at 650 nm. Values were determined according to a standard curve (log/log curve-fit). View the Molecular Devices website. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. PC12 or BV-2 cells treated with various concentrations of BBD were stressed with hypoxia for one hour and the supernatant was then assayed for LDH activity. Samples were analyzed in triplicates. Permeability of the monolayer was verified by adding 1% Evans Blue dye (Thermo Fisher Scientific, Inc. The adapter is. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Buffer (100 mmol L –1acetate buffer, pH 5. P. Unit powers. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). Call 1-800-745-2710. After 72 h, 20 μl of 5 mg/ml MTT solution was added to each well and plates were incubated for 3 h at 37°C. Cell Culture AO391 (derived from human aorta) and CD314 (derived from human carotid artery) cell lines were a generous gift from Wendy Bauer (Tufts Molecular Cardiology Research Institute) and were grown in DMEM (CellGro) supplemented with 10% FBS. View the Molecular Devices website. The SpectraMax i3x reader provides the flexibility to add novel detection capabilities without the need for service engineers or costly downtime—it grows with you as your research areas expand. SpectraMax® Microplate Readers and SoftMax® Pro Software. 340/80 nm excitation with 616/10 nm emission range Optimized for use with ScanLater Western Blot Detection cartridge for the SpectraMax® i3, i3x, and Paradigm® Multi-Mode Readers Optimized for use with the. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. , Sunnyvale, CA). TDP-43 stress granular cells were treated with 70% ice-cold. Manager: Yinghua Chen, PhD Contact Email: yinghua. 3. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). Labsystems Luminoskan RT Microplate Reader. SpectraMax Microplate Readers. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. 3 0. Introduction. 3: The cuvette chamber. The cell survival. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). The ODs of wells loaded with DMEM plus 10% CS were subtracted as background. Unit has several areas of damaged plastic. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Data were expressed as the mean percent viable cell vs. 2 M DEEMM were mixed in a microtube,. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. The optical system of the SpectraMax 340PC Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). Operators can conveniently select up to 6 wavelengths at a time for. Absorbance was read at 405 nm on a microplate reader (SPECTRAmax 340 PC). SpectraMax 384 Plus. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. (1988) and recorded at 412 nm. After adding sample, the mixture was incubated for 30 min at 37°C, with one unit of enzymatic activity being defined as an increase in absorbance of 0. These isotypes cause antigen cross-linking by multimeric binding and self. The cell proliferation on different Ti discs was assessed by measuring the absorbance at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Devices, Sunnyvale, CA). The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. 312 gain calibration failed. Male 8 pin mini Din to Female 9D. \r plates can be used in the SPECTRAmax PLUS 384. Spectrophotometer (SpectraMax 340). The sensitivities of the hormones are Prolactin: 0. Heme was preincubated for 5 min with A when added before the metals. 5. The results are expressed as the percentage inhibition of BrdU incorporation compared to the untreated control. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Data are expressed as percent of maximum binding for each protein. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. Time-resolved fluorescence was measured on the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). The inhibition percentage was determined by linear regression through ranges of 20 to 30. Samples with OD values of less than 0. Lactate dehydrogenase (LDH) release assay Cytotoxicity was determined by measuring the release of LDH. Integration delay.